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GC00181-SD.C9orf72(tm)-GC/ILAS-C9orf72-313155-Knockout-Live
来源: | 作者:moxingzhongxin | 发布时间: 2020-04-09 | 143 次浏览 | 分享到:
GC00181

Accession No

GC00181

Strain Name

SD.Epha4(tm-mcherry)-GC/ILAS

Status

Live

Gene/full name

Epha4

Gene ID

316539

Background

Sprague Dawley

Type

Knockin

Donor

Lianfeng Zhang, Wei Dong, Li Zhang, Xiang Gao, Wei Chen

Disease & Phenotype

Amyotrophic lateral sclerosis (ALS)

Cardiovascular disease

Construction Description

Genotyping Protocols

敲除检测

R-EPHA4-mcheery-up-F  

5’tgatttcacccttcgcagtc     

R-EPHA4-mcheery-up-R  

5’TTACTTGTACAGCTCGTCCATGC  

R-EPHA4-mcheery-down-F  

5’GAGGCTGAAGCTGAAGGACG          

R-EPHA4-mcheery-down-R  

5’TCCTTACAGACTAGTTCACACCATTCC  

R-EPHA4-mcherry-wt-F 

5’CTGGGATAGAAGCGGTAGGAG    

R-EPHA4-mcherry-wt-R 

5’CTGAGCTCCCGAACCATTC     

Up: 1514bp,  down: 1502bp,  WT: 208bp

Mating System

Heterozygote x Heterozygote;

Homozygote is fertile

Reference