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GC00186-SD.C9orf72(tm-HRE)-GC/ILAS-C9orf72-313155-Knockin
来源: | 作者:moxingzhongxin | 发布时间: 2020-04-09 | 255 次浏览 | 分享到:
GC00186

Accession No

GC00186

Strain Name

SD.C9orf72(tm-HRE)-GC/ILAS

Status

Live

Gene/full name

C9orf72

Gene ID

313155

Background

Sprague Dawley

Type

Knockin

Donor

Lianfeng Zhang, Wei Dong, Li Zhang, Xiang Gao, Wei Chen

Disease & Phenotype

Amyotrophic lateral sclerosis (ALS)

 

Construction Description

Genotyping Protocols

检测引物:

R-C9-HGC-WT-F1  

5’TGTAGTGTCAGCCATCCCAATTG       

R-C9-HGC-WT-R1  

5’GGAACAGTGTGACTAGAAATTTATCCACC  

R-C9-HGC-up-F1    

5’TAAGAACTTAACAGATGACAGTTGCTGG  

R-C9-HGC-up-R1    

5’TTCTTGTTCACCCTCAGCGAG          

R-C9-HGC-down-F1  

5’TTTACTTTCCCTCTCATTTCTCTGACC     

R-C9-HGC-down-R1  

5’GGAACAGTGTGACTAGAAATTTATCCACC   

WT1084bp,   up965bp     down809bp

 

Mating System

Heterozygote x Heterozygote;

Homozygote is fertile

Reference