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GC00014

来源: 作者: 发布时间: 2021-05-08 466 次浏览

Accession No

GC00014

Strain Name

SD.Tg(BAC-h-Abcb1)-GC/ILAS

Status

Freezed embryo

Gene/full name

Abcb1b /

ATP-binding cassette, subfamily B (MDR/TAP), member 1B

Gene ID

24646

Background

Sprague Dawley

Type

Transgene

Donor

Lianfeng Zhang, Yuanwu Ma,Jing Ma, Xu Zhang, Wei Chen

Disease & Phenotype

drug metabolism

 

Construction Description

Genotyping Protocols

BAC    Tm=58℃   Fragment:350bp

Former   CACCAGTTGAAGAGCGTTGA

Reverse   GCTGCTGATGTGCTGATTGT

 

MDR     Tm=58℃   Fragment:410bp

Former4222   AGTGGTGTTTCAGAATGGC

Reverse4631   GTCAGTTACAGTCCAAATGGG

 

MDR-3 UTR  Tm=56℃  Fragment:448bp

Former2948   CCAATGATGCTGCTCAAG

Reverse3395   GAGTTTATGTGCCACCAAGTAG

 

 

Mating System

Hemizygous x Background(SD);

 

Reference

 

1.马婧,陈炜,张旭.多药耐药基因1(Abcb1)敲除和人源化大鼠模型的建立,《中国比较医学杂志》,2015.