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GC00010

来源: 作者: 发布时间: 2021-05-08 394 次浏览

Accession No

GC00010

Strain Name

SD.Prkdc(tm)-GC/ILAS

Status

Freezed embryo

Gene/full name

Prkdc/protein kinase,

DNA-activated, catalytic subunit

Gene ID

360748

Background

Sprague Dawley

Type

Knockout

Donor

Lianfeng Zhang, Yuanwu Ma, Xu Zhang, Wei Chen

Disease & Phenotype

immune system diseases

Construction Description

Genotyping Protocols

Prkdc-F  AGATGCACTTGGGTAAACACCTG

Prkdc-R  GTAAGTGACAGGTATCTCACCAG

 

Mating System

Heterozygote x Heterozygote;

SPF (specific pathogen-free) conditions are recommended.

Reference

 

  1. Yuanwu Ma,Bin Shen,Xu Zhang. Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9, PLoS One. 2014; 9(3): e89413..