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GC00201

来源: 作者: 发布时间: 2021-11-05 251 次浏览

GC00201​

SD.MT-Trn K(G7755A)-GC/ILAS

Accession No

GC00201

Strain Name

SD.MT-Trn K(G7755A)-GC/ILAS

Status

Frozen Embryo

Gene/full name  

MT-Trn K

Gene ID  

170615

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen Lianfeng Zhang

Disease & Phenotype  

MERRF, cardiomyopathy and Leigh Syndrome

Construction Description

 

Genotyping Protocols

Mutation Detection Primer

FTGCTCTGAAATTTGCGGCTC

RAGTCGGTTGCTGATTAGGCG

Standard PCR

PCR条件:945min;(9420s, 6030s, 721min) 30 cycle; 7210min; 4 

Mating System

Mutant ratcross with wildtype SD rat;

Maternal inherited

Reference

Qi, X., Chen, X., Guo, J. et al. Precision modeling of mitochondrial disease in rats via DdCBE-mediated mtDNA editing. Cell Discov 7, 95 (2021).