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GC00209

来源: 作者: 发布时间: 2022-11-01 42 次浏览

GC00209

SD. MT-Trn E(G14098A)/MT-Trn K(G7755A)-GC-ILAS

Accession No

GC00209

Strain Name

SD. MT-Trn E(G14098A)/MT-Trn K(G7755A)-GC-ILAS

Status

Frozen Embryo

Gene/full name  

MT-Trn E; MT-Trn K

Gene ID  

170619; 170615

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen Lianfeng Zhang

Disease & Phenotype  

Mitochondrial Disease

Construction Description

Genotyping Protocols

突变检测引物:

7755-FTGCTCTGAAATTTGCGGCTC

7755-RAGTCGGTTGCTGATTAGGCG

14098-FTCAAGTCTCCGGGTACTCCT

14098-RAAATATTGAGGCGCCGTTGG

Standard PCR

PCR条件:945min;(9420s, 6030s, 721min) 30 cycle; 7210min; 4 

Mating System

Mutant ratcross with wildtype SD rat;

Maternal inherited

Reference