新闻资讯

以真诚努力,服务医学进步

GC00210

来源: 作者: 发布时间: 2022-11-01 33 次浏览

GC00210

SD.MT-Trn F(G007A)-GC/ILAS

Accession No

GC00210

Strain Name

SD.MT-Trn F(G007A)-GC/ILAS

Status

Frozen Embryo

Gene/full name  

MT-Trn F

Gene ID  

170599

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen Lianfeng Zhang

Disease & Phenotype  

mitochondrial myopathy

Construction Description

Genotyping Protocols

突变检测引物:

FAGGCATCTGGTTCTTACTTCAGG

RTTATTAAGGTTTAGGGCTAAGCATAGTGG

WT     G007A

 

Standard PCR

PCR条件:94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Mutant ratcross with wildtype SD rat;

Maternal inherited

Reference