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GC00213

来源: 作者: 发布时间: 2022-11-01 47 次浏览

GC00213

SD.MT-Trn V(G1030A/G1032A)-GC/ILAS

Accession No

GC00213

Strain Name

SD.MT-Trn V(G1030A/G1032A)-GC/ILAS

Status

Frozen Embryo

Gene/full name  

MT-Trn V

Gene ID  

170601

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen Lianfeng Zhang

Disease & Phenotype  

Mitochondrial Disease

Construction Description

 

Genotyping Protocols

突变检测引物:

FGCGGTACTTTATATCCATCTAGAGG 

RGTTTTTAGGTAGCTCGTTTGGTTTC

WT           G1030A-G1032A

 

Standard PCR

PCR条件:945min;(9420s, 6030s, 721min) 30 cycle; 7210min; 4 

Mating System

Mutant ratcross with wildtype SD rat;

Maternal inherited

Reference