新闻资讯

以真诚努力,服务医学进步

GC00214

来源: 作者: 发布时间: 2022-11-01 68 次浏览

GC00214

SD.MT-Trn F(G007A)/MT-Trn V(G1030A)/MT-Trn L2(G11714/5A)-GC/ILAS

Accession No

GC00214

Strain Name

SD.MT-Trn F(G007A)/MT-Trn V(G1030A)/MT-Trn L2(G11714/5A)-GC/ILAS

Status

Live

Gene/full name  

MT-Trn F; MT-Trn V; MT-Trn L2

Gene ID  

170599; 170601; 359842

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen Lianfeng Zhang

Disease & Phenotype  

mitochondrial myopathy

Construction Description

 

Genotyping Protocols

突变检测引物:

G007-FAGGCATCTGGTTCTTACTTCAGG

G007-RTTATTAAGGTTTAGGGCTAAGCATAGTGG 

G1030-FGCGGTACTTTATATCCATCTAGAGG

G1030-RGTTTTTAGGTAGCTCGTTTGGTTTC

G11714/5-FTGAATCTAACAACAGGAAATCAAATTC

G11714/5-RGATATAATTCCTACTCCTTCTCATCCAAT

 

 

 

 

WT     Mut

 

 

Standard PCR

PCR条件:94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Mutant ratcross with wildtype SD rat;

Maternal inherited

Reference