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GC00215

来源: 作者: 发布时间: 2022-11-01 36 次浏览

GC00215

SD. Rosa26(tm-CAG-LSL-MT-ND1(G2996A))-GC/ILAS

Accession No

GC00215

Strain Name

SD. Rosa26(tm-CAG-LSL-MT-ND1(G2996A))-GC/ILAS

Status

Live

Gene/full name  

MT-Nd1

Gene ID  

26193

Background

Sprague Dawley

Type

Knock in

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen Lianfeng Zhang

Disease & Phenotype  

Mitochondrial Disease

Construction Description

 

Genotyping Protocols

Primers

TG-F: TTGCGGAGACTCACCCCTT

TG-R:TGTAAACCCATGGCCGAC

2996-FGAAAATCCTAGGCTACATACAACTACG

2996-RGAGGATAATGGCTATTGTGACTT

Standard PCR

PCR条件:PCR条件:94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Cross with wildtype SD rat

Reference